These new projects are dealing with the use of DNA microarrays to define genes relevant for tumor biology and clinical outcome. A central gene chip facility has been established at our department in which a DNA microarray scanner and the necessary software and computers for analysis of microarrays are available. The colon tumor project is a collaborative effort within the National Genome Research Network (NGFN) and mainly carried out by several clinical groups in collaboration with our group. The renal carcinoma project is mainly worked out in our group in close collaboration with the Urology department at the Waldkrankenhaus (PD Dr. Z. Akcetin).
Renal epithelial neoplasms are increasingly common making up 2 % of all adult malignancies today. In 30 % of the patients metastasis in lung, skeleton, liver or brain are already present at the time of diagnosis. Of the remaining patients with initially no metastasis, 30 – 50 % relapse after surgery and usually succumb to the disease. It is currently not possible to predict which tumors metastasize into the lymph nodes or distant organs, especially, because of the lack of understanding of the underlying molecular mechanisms that may cause this variety in prognosis.
Therefore we are performing a global gene expression analysis on renal clear cell carcinomas using DNA microarrays to identify marker genes or gene clusters which are relevant for tumor biology and the clinical outcome. So far we have established expression profiles of several kidney tumor samples and compared them to those of normal kidney tissue by Affymetrix GeneChip analysis. We could already detect various genes up- or downregulated in tumors and are currently extending this analysis (M. Sachs, J. Behrens). Source : Department of Experimental Medicine II – Exp2med
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